generate.red.bkg: Get background red fluorescence for induction timecourses
In ctriandafillou/flownalysis: Analysis of Yeast Protein Induction and pH by Flow Cytometry
Description
Usage
Arguments
Value
View source: R/generate.red.bkg.R
Description
From a dataframe representing a stress and recovery experiment (timecourse measuring induction of a red fluorophore-tagged protein), generate the background level of fluorescence in untreated cells.
Usage
1
generate.red.bkg(df, experiment.name = FALSE, media = "none")
Arguments
df
Name of the dataframe which contains the induction data. Should be an output of read.chicago.flowSet or similar formatting.
experiment.name
The precise name of the experiment to use to generate the background. Will be in the 'exp' column. Default value is FALSE, where the experiment name will be inferred.
media
Is the background acidic or buffered neutral SC? If so, specify 'sc' or 'buffered.sc'; otherwise returns the median of all rows which have 'backgrounds' and 'ycgt028' in the 'exp' column.
Value
A single numerical value which is the median of the distribution of size-normalized red fluroescence per cell (PEDazzle area / forward scatter area).
ctriandafillou/flownalysis documentation built on Sept. 7, 2020, 1:48 a.m.
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Description Usage Arguments Value
View source: R/generate.red.bkg.R
Description
From a dataframe representing a stress and recovery experiment (timecourse measuring induction of a red fluorophore-tagged protein), generate the background level of fluorescence in untreated cells.
Usage
1 | generate.red.bkg(df, experiment.name = FALSE, media = "none")
|
Arguments
df |
Name of the dataframe which contains the induction data. Should be an output of read.chicago.flowSet or similar formatting. |
experiment.name |
The precise name of the experiment to use to generate the background. Will be in the 'exp' column. Default value is FALSE, where the experiment name will be inferred. |
media |
Is the background acidic or buffered neutral SC? If so, specify 'sc' or 'buffered.sc'; otherwise returns the median of all rows which have 'backgrounds' and 'ycgt028' in the 'exp' column. |
Value
A single numerical value which is the median of the distribution of size-normalized red fluroescence per cell (PEDazzle area / forward scatter area).
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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