Description Usage Arguments Examples
Make gRNAs from GeCKO Library
1 2 | make_gRNAs(seqs, gene = NULL, seq1_5p = "CACCG", seq1_3p = "",
seq2_5p = "AAAC", seq2_3p = "C")
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gene |
1 2 3 4 5 6 7 8 9 10 11 12 13 | # Make oligos from a single sequence
seq <- "TTCCTCACCTGATGATCTTG"
gene <- "GAPDH"
make_gRNAs(seq, gene)
# Make oligos for multiple gRNA sequences
human <- get_library(species = 'human')
seq <- human[gene_id=='GAPDH', seq]
gene <- human[gene_id=='GAPDH',gene_id]
make_gRNAs(seqs = seq, gene = gene)
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