Lamelli SDS PAGE is cast using 5 components (30% Acrylamide solution, Tris-HCl Buffer, Water, TEMED and 10% APS). The Gelcast function calculates and prints the volumes of these components to be mixed, to cast a particular volume and percentage of running and stacking gels.
1 | Gelcast(runvol, runpc, stkvol, stkpc)
|
runvol |
a numeric value representing the final volume of running gel mix required. |
runpc |
a numeric value representing the running gel percentage required. |
stkvol |
a numeric value representing the final volume of stacking gel mix required. |
stkpc |
a numeric value representing the stacking gel percentage required. |
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