FindsgRNAfunction | R Documentation |
Warning: This function should not be directly called by the user - it must be called though RunShiny.R
Designs sgRNA based on inputs provided in the Shiny App.
sgRNA_design_function(userseq, genomename, gtf,
designprogress, userPAM, calloffs, annotateoffs)
userseq |
The target sequence to generate sgRNA guides for. Can either be a character sequence containing DNA bases or the name of a fasta file in the working directory. |
genomename |
The name of a geneome (from the BSgenome package) to check off-targets for. |
gtf |
The name of a genome annotation file (.gtf) in the working directory to check off-target sequences against. |
designprogress |
Assists in communicating the progress of the sgRNA design to the Shiny App. |
userPAM |
An optional argument used to set a custom PAM for the sgRNA. If not set, the function will default to the "NGG" PAM. Warning: Doench efficieny scores are only accurate for the "NGG" PAM. |
calloffs |
If TRUE, the function will search for off-targets in the genome chosen specified by the genomename argument. If FALSE, off-target calling will be skipped. |
annotateoffs |
If TRUE, the function will provide annotations for the off-targets called using the genome annotation file specified by the gtfname argument. If FALSE, off-target annotation will be skipped. |
A list containing all data on the generated sgRNA and all off-target information. List items 1 through 15 include information on each individual sgRNA, including the sgRNA sequence itself, PAM, location, direction relative to the target sequence, GC content, homopolymer presence, presence of self-complementarity, off-target matches, predicted efficiency score, and a notes column that summarizes unfavorable sequence features. List items 16 through 27 include all information on off-target matches, including the original sgRNA sequence, off-target sequence, chromosome, location, direction relative to the target sequence, number of mismatches, gene ID, gene name, type of DNA, and exon number.
Dylan Beeber
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