measure_dna_concentration: Measure DNA concentration for using qubit to quantify DNA

In econtijoch/FaithLabTools: R Scripts for Faith Lab @ MSSM

Measure DNA concentration for using qubit to quantify DNA

Description

Measure DNA concentration for using qubit to quantify DNA

Usage

measure_dna_concentration(plate_reader_file,
  standards_plate_reader_file = plate_reader_file, standard_wells, dye_used,
  qubit_volume = 2, elution_volume = 100, plate_size = 96,
  plate_name = "DNA_Plate", print_standard_curve = FALSE,
  omit_standards = NULL)

Arguments

plate_reader_file	relative location to relevant plate reader file (accepts excel files)
standards_plate_reader_file	relative location to the plate reader file containing the standards (if it is the same as the plate you want to quantify, it is not necessary to explicitly specify
standard_wells	a vector containing the wells that contain the standards in increasing concentration (e.g. c("A01", "A02", "A03", "A04", "A05", "A06", "A07", "A08")) - needs to be sequential. If you need to exclude any standards, use omit_standards
dye_used	"BR" or "HS" to specify quantification with HS or BR dye
qubit_volume	volume of sample used for quantification (default = 2)
elution_volume	volume of your sample (used to compute total DNA in sample, default = 100)
plate_size	size of plate (default = 96, alternative is 384 - not tested yet)
plate_name	name to give plate (helps when using multiple plates)
print_standard_curve	TRUE or FALSE to indicate whether or not to save a .pdf image containing the standard curve
omit_standards	pass along a well location to exclude from standard analysis (helpful for when one fails)

Value

table with DNA concentrations for each sample, given the input parameters

econtijoch/FaithLabTools documentation built on July 21, 2022, 8:34 a.m.