qPCR: Function to automate the analysis of qPCR data

In funkhou9/molbio: Analysis of common experimental data

Description

Designed for tabular qPCR data of a specific format generated by the Ernst Lab at Michigan State University. To see an example of the data, load an example external dataset with data("adar_data").

Usage

qPCR(data, target, reference, calibrator, rm_files = "none", text_size = 15,
  orders = NULL, background = TRUE)

Arguments

data	data.frame of tabular qPCR data
calibrator	character with name of sample used for calibration
rm_files	character vector containing names of samples to remove
text_size	numeric specifying the size of the axis and axis lables
background	logical indicating if traditional ggplot gray background is desired. If FALSE, a white background with horizontal lines is used
target_gene	character with name of target gene used in analysis
ref_gene	character with name of reference gene used in analysis
order	character vector specifying the order that the tissues appear in the resulting plot

Value

a new data.frame with same fields but added "ratios" column to provide fold-expression changes relative to calibrator

funkhou9/molbio documentation built on May 16, 2019, 4:05 p.m.