pcr_profile: Creates a thermocycler profile for PCR
In grunwaldlab/labtools: Lab Notebook Tools

Description Usage Arguments See Also

Creates a thermocycler profile for PCR in either graphical or verbal form. This is intended to be used for mostly generic profiles. For more advanced profiles, use thermocycler_profile. All temperature and time parameters take numeric inputs in celsius and seconds respectively.

1
2
3

pcr_profile(cycles = 30, init_tm = 95, init_time = 300, denat_tm = 96,
  denat_time = 25, anneal_tm = 55, anneal_time = 30, elong_tm = 72,
  elong_time = 100, final_tm = 72, final_time = 600, hold_tm = 5)

`cycles`	(`integer`) The number of cycles.
`init_tm`	(`numeric`) The initial heating temperature used before cycling starts.
`init_time`	(`numeric`) The duration of the initial heating used before cycling starts.
`denat_tm`	(`numeric`) The temperature of the denaturation step.
`denat_time`	(`numeric`) The duration of the denaturation step.
`anneal_tm`	(`numeric`) The temperature of the annealing step.
`anneal_time`	(`numeric`) The duration of the annealing step.
`elong_tm`	(`numeric`) The temperature of the elongation step.
`elong_time`	(`numeric`) The duration of the elongation step.
`final_tm`	(`numeric`) The temperature of the final elongation step used after cycling completes.
`final_time`	(`numeric`) The duration of the final elongation step used after cycling completes.
`hold_tm`	(`numeric`) The temperature used after all other steps to hold the sample.