normalize_counts | R Documentation |
Total count normalization of DNA and RNA counts.
normalize_counts(object, block = NULL)
object |
An object of class |
block |
A vector giving the sample designations of the columns of
|
block
is a vector that is used when the columns of the
MPRAset
object are paired. This often is the case when comparing
allelic versions of an element. In this case, the first $s$ columns of
object
give the counts for the reference allele in $s$
samples. The second $s$ columns give the counts for the alternative
allele measured in the same $s$ samples. With 3 samples, block
would be c(1,2,3,1,2,3)
. All columns are scaled to have 10
million counts.
An object of class MPRASet
with the total count-normalized DNA
and RNA counts.
data(mpraSetAggExample)
mpraSetAggExample <- normalize_counts(mpraSetAggExample)
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