Description Usage Arguments Value Examples
Parse the read-depth information of a VCF file produced by mpileup
1 | parse_vcf(fname)
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fname |
path to the file |
a tibble
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 | ## Not run:
# head of the VCF file
#> ##fileformat=VCFv4.2
#> ##FILTER=<ID=PASS,Description="All filters passed">
#> ##bcftoolsVersion=1.9+htslib-1.9
#> ##bcftoolsCommand=mpileup -Ov --annotate INFO/AD,FORMAT/AD,FORMAT/DP ...
#> ##reference=file:///n/no_backup2/dbmi/park/jc604/databases/reference_...
#> ##contig=<ID=chr1,length=248956422>
#> ##contig=<ID=chr2,length=242193529>
#> ##contig=<ID=chr3,length=198295559>
#> ##contig=<ID=chr4,length=190214555>
#> ##contig=<ID=chr5,length=181538259>
#> ##contig=<ID=chr6,length=170805979>
#> ##contig=<ID=chr7,length=159345973>
#> ##contig=<ID=chr8,length=145138636>
#> ##contig=<ID=chr9,length=138394717>
#> ##contig=<ID=chr10,length=133797422>
#> ##contig=<ID=chr11,length=135086622>
#> ##contig=<ID=chr12,length=133275309>
#> ##contig=<ID=chr13,length=114364328>
#> ##contig=<ID=chr14,length=107043718>
#> ##contig=<ID=chr15,length=101991189>
#> ##contig=<ID=chr16,length=90338345>
#> ##contig=<ID=chr17,length=83257441>
#> ##contig=<ID=chr18,length=80373285>
#> ##contig=<ID=chr19,length=58617616>
#> ##contig=<ID=chr20,length=64444167>
#> ##contig=<ID=chr21,length=46709983>
#> ##contig=<ID=chr22,length=50818468>
#> ##contig=<ID=chrX,length=156040895>
#> ##contig=<ID=chrY,length=57227415>
#> ##contig=<ID=chrM,length=16569>
#> ##contig=<ID=chr1_KI270706v1_random,length=175055>
#> ##contig=<ID=chr1_KI270707v1_random,length=32032>
#> ##contig=<ID=chr1_KI270708v1_random,length=127682>
#> ...
#> ##INFO=<ID=QS,Number=R,Type=Float,Description="Auxiliary tag used for calling">
#> ##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
#> ##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Number of high-quality bases">
#> ##FORMAT=<ID=AD,Number=R,Type=Integer,Description="Allelic depths">
#> ##INFO=<ID=AD,Number=R,Type=Integer,Description="Total allelic depths">
#> #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT TCGA-FZ-5924-01A-13D-1609-08
#> chr12 25202434 . A <*> 0 . DP=1;AD=1,0;I16=1,0,0,0,29,841,0,0,60,3600,0,0,0,0,0,0;QS=1,0;MQ0F=0 PL:DP:AD 0,3,29:1:1,0
#> chr12 25202435 . G <*> 0 . DP=1;AD=1,0;I16=1,0,0,0,31,961,0,0,60,3600,0,0,1,1,0,0;QS=1,0;MQ0F=0 PL:DP:AD 0,3,31:1:1,0
#> chr12 25202436 . C <*> 0 . DP=1;AD=1,0;I16=1,0,0,0,28,784,0,0,60,3600,0,0,2,4,0,0;QS=1,0;MQ0F=0 PL:DP:AD 0,3,28:1:1,0
#> chr12 25202437 . A <*> 0 . DP=1;AD=1,0;I16=1,0,0,0,30,900,0,0,60,3600,0,0,3,9,0,0;QS=1,0;MQ0F=0 PL:DP:AD 0,3,30:1:1,0
#> chr12 25202438 . G <*> 0 . DP=1;AD=1,0;I16=1,0,0,0,34,1156,0,0,60,3600,0,0,4,16,0,0;QS=1,0;MQ0F=0 PL:DP:AD 0,3,34:1:1,0
#> chr12 25202439 . G <*> 0 . DP=1;AD=1,0;I16=1,0,0,0,33,1089,0,0,60,3600,0,0,5,25,0,0;QS=1,0;MQ0F=0 PL:DP:AD 0,3,33:1:1,0
#> ...
parse_vcf("example.vcf")
#> # A tibble: 8,917 x 7
#> start ref alt qual read_info num_high_qual_bas<e2><80><a6> allelic_depths
#> <dbl> <chr> <chr> <dbl> <chr> <chr> <chr>
#> 1 25202434 A <*> 0 0,3,29:1:1,0 1 1
#> 2 25202435 G <*> 0 0,3,31:1:1,0 1 1
#> 3 25202436 C <*> 0 0,3,28:1:1,0 1 1
#> 4 25202437 A <*> 0 0,3,30:1:1,0 1 1
#> 5 25202438 G <*> 0 0,3,34:1:1,0 1 1
#> 6 25202439 G <*> 0 0,3,33:1:1,0 1 1
#> 7 25202440 G <*> 0 0,3,34:1:1,0 1 1
#> 8 25202441 G <*> 0 0,3,33:1:1,0 1 1
#> 9 25202442 A <*> 0 0,3,32:1:1,0 1 1
#> 10 25202443 T <*> 0 0,3,31:1:1,0 1 1
#> # <e2><80><a6> with 8,907 more rows
## End(Not run)
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