calc.genoprob: Calculate conditional genotype probabilities

In kbroman/qtl: Tools for Analyzing QTL Experiments

Calculate conditional genotype probabilities

Description

Uses the hidden Markov model technology to calculate the probabilities of the true underlying genotypes given the observed multipoint marker data, with possible allowance for genotyping errors.

Usage

calc.genoprob(cross, step=0, off.end=0, error.prob=0.0001,
              map.function=c("haldane","kosambi","c-f","morgan"),
              stepwidth=c("fixed", "variable", "max"))

Arguments

cross	An object of class cross. See read.cross for details.
step	Maximum distance (in cM) between positions at which the genotype probabilities are calculated, though for step = 0, probabilities are calculated only at the marker locations.
off.end	Distance (in cM) past the terminal markers on each chromosome to which the genotype probability calculations will be carried.
error.prob	Assumed genotyping error rate used in the calculation of the penetrance Pr(observed genotype | true genotype).
map.function	Indicates whether to use the Haldane, Kosambi or Carter-Falconer map function when converting genetic distances into recombination fractions.
stepwidth	Indicates whether the intermediate points should with fixed or variable step sizes. We recommend using "fixed"; "variable" was included for the qtlbim package (https://cran.r-project.org/src/contrib/Archive/qtlbim/). The "max" option inserts the minimal number of intermediate points so that the maximum distance between points is step.

Details

Let O_k denote the observed marker genotype at position k, and g_k denote the corresponding true underlying genotype.

We use the forward-backward equations to calculate \alpha_{kv} = \log Pr(O_1, \ldots, O_k, g_k = v) and \beta_{kv} = \log Pr(O_{k+1}, \ldots, O_n | g_k = v)

We then obtain Pr(g_k | O_1, \ldots, O_n) = \exp(\alpha_{kv} + \beta_{kv}) / s where s = \sum_v \exp(\alpha_{kv} + \beta_{kv})

In the case of the 4-way cross, with a sex-specific map, we assume a constant ratio of female:male recombination rates within the inter-marker intervals.

Value

The input cross object is returned with a component, prob, added to each component of cross$geno. prob is an array of size [n.ind x n.pos x n.gen] where n.pos is the number of positions at which the probabilities were calculated and n.gen = 3 for an intercross, = 2 for a backcross, and = 4 for a 4-way cross. Attributes "error.prob", "step", "off.end", and "map.function" are set to the values of the corresponding arguments, for later reference (especially by the function calc.errorlod).

Author(s)

Karl W Broman, [e-mail]

References

Lange, K. (1999) Numerical analysis for statisticians. Springer-Verlag. Sec 23.3.

Rabiner, L. R. (1989) A tutorial on hidden Markov models and selected applications in speech recognition. Proceedings of the IEEE 77, 257–286.

See Also

sim.geno, argmax.geno, calc.errorlod

Examples

data(fake.f2)
fake.f2 <- calc.genoprob(fake.f2, step=2, off.end=5)

data(fake.bc)
fake.bc <- calc.genoprob(fake.bc, step=0, off.end=0, err=0.01)

kbroman/qtl documentation built on Aug. 26, 2024, 12:33 a.m.