RacepcR: Race PCR Primer Designer

In lrjoshi/RacepcR:

Description

You can use this package to design 3' and 5'primers for Race PCR. The design is based on as described by Clonetech Race PCR manual. The resulting primers will have vector sequence automatically added to them.

Usage

getDNA(dnaseq)
quality (dnaseq)
gcbarplot(dnaseq)
get5prime(dnaseq,x)
get3prime(dnaseq,y)

Arguments

dnaseq	it is the sequence of your DNA. For eg : "aaggcctt"
x	position where you want to start your 5' primer
y	position where you want to start your 3' primer

Note

This package depends on Biostrings package. So, you should have already installed Biostrings package from Bioconductor.

See Also

https://github.com/lrjoshi/RacepcR

Examples

library (RacepcR)
library(Biostrings)
#function collection
getDNA("aaggccttcc")
quality ("aaggcct")
gcbarplot("aaaaagccggt")
get5prime("aaaggcggcggcttcggcgcgctcgtgctgctgctgcggcgctcctttcgctcgtcgctgctttctctcgct",30)
get3prime("aaaggcggccccctcttcggccttcgctgctgctgcggcgcggctcgcgctgctgctttgctgctcgggctcgt",10)

lrjoshi/RacepcR documentation built on May 26, 2019, 6:35 a.m.