Description Usage Arguments Value Note
Extracting fluorescence values Extracting fluorescence values in an .h5 file in the BigDataViewer format around given coordinates
1 2 | getFluo(H5_path, Spots_df, x_px = x_px, y_px = y_px, z_px = z_px,
timepoint = FRAME, cubeSize = 5)
|
H5_path |
path to the H5 file used to create the mamut.xml file |
Spots_df |
dataframe containing on each rows spots locations at which to retrieve fluorescent values |
x_px |
name of the column with x coordinates in pixels in Spots_df |
y_px |
name of the column with y coordinates in pixels in Spots_df |
z_px |
name of the column with z coordinates in pixels in Spots_df |
timepoint |
name of the column where the timepoints or frames are stored |
cubeSize |
half the length of the cube side length. By default the cube is 10x10x10 pixels. |
a list of length equal to the number of timepoints. For each timepoint, values of fluorescence around each spot location
Many thanks to Dr. Mike Smith for his help on this function.
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