hitachi_tibble: hitachi_tibble

In mirkko-hub/hitac2:

Description

takes left_join(hitchi_read / hitachi_read_xls, design, by = Hitachi_ID) as input and does background subtraction and sa-calculation on basis of provided parameters V_RuBP and C_RuBP. If provided, adds additional groupwise information by left_join(., treatment, by = 'Group').

Usage

hitachi_tibble(
  data,
  treatment,
  Join_bg = Join_bg,
  Join_sa = Join_sa,
  V_RuBP = 4e-06,
  C_RuBP = 6e-04,
  convert = TRUE
)

Arguments

data	left_join(hitchi_read / hitachi_read_xls, design, by = Hitachi_ID) as input. Minimum columns 'Group' and 'CPM'.
treatment	additional file providing groupwise information, e.g substrate concentration. Joined with data by column 'Group'.
Join_bg	default to column name 'Join_bg', provides ties for background counts (e.g. dbl values).
Join_sa	default to column name 'Join_sa', provides ties for specific activity (sa) counts (e.g. dbl values).
V_RuBP	Volume [L] of RuBP-solution added to sa-reactions - default to 0.000004 .
C_RuBP	Concentration [M] of RuBP-solution added to sa-reactions - default to 0.0006 .
convert	default to T - converts CPM in Activity_nmol fixed carbon after groupwise background subtraction (Group == "control"). Requires parameters V_RuBP and C_RuBP!

Examples

hitachi_tibble()

mirkko-hub/hitac2 documentation built on Jan. 1, 2021, 2:53 p.m.