jasco_extract_params: jasco_extract_params

In mirkko-hub/jasco2: Handling of Jasco Spectrophotometer data

Description

extracts turnover of ATP molecules per protein (min^-1). For input, see examples or README.

Usage

jasco_extract_params(.data, .bgcorr, .protein_uM, .unit, .factor, ...)

Arguments

.data	a tibble, minimal column 'rate_avg'
.bgcorr	optional background correction (subtraction); NADH oxidation in absence of protein
.protein_uM	final protein concentration in assay
.unit	current time scale; one of h = hour, min = minutes, sec = seconds, msec = milliseconds
.factor	coupling factor; eg. for ATPase assay NADH decline is monitored: each oxidized NADH molecule reports the hydrolysis of one molecule of ATP; hence, coupling factor is '-1'
.group_vars	grouping variable for summary report (eg. 'Group' - for grouping replicates)

Value

list of two tibbles; 'data' and 'summary'. 'data' contains all columns, including model statistics for evaluation. 'summary' is a grouped summary of turnover(min^-1) ('turnover_avg') and sd ('turnover_sd')

Examples

# typical output from jasco_tibble()
jasco_df

jasco_df %>%
  convert_absorbance(., "NADH") %>%
  jasco_extract_lm(., response = NADH, predictor = Time_s, min = 75, max = 175) %>%
  jasco_extract_params(., .bgcorr = FALSE, .protein_uM = 6, .unit = "sec", .factor = -1, Group)

mirkko-hub/jasco2 documentation built on Jan. 1, 2021, 2:53 p.m.