gcount
is an R-package that allows to obtain raw read counts
quickly and easily from RNASeq data to be used in downstream analyses.
The current functionality is as follows:
Provide a bam/bed
file or object along with gtf/gff
file or object as input.
Specify sequencing type: paired
or single
end data.
Specify library type: unstranded
, first-strand
or
second-strand
specific.
Filter reads based on number of mismatches prior to counting if necessary.
Consider or ignore reads that map to overlapping genes.
Count reads that overlap feature of interest – genes
,
exons
, introns
etc.
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