dCt2: Delta Ct calculation

In rhecht95/qPCR: Data Wrangling and Analysis for qPCR Data Generated by QuantStudio

Description

Subtracts Ct of housekeeping gene from Ct of experimental target and returns values as a new column to input data frame

Usage

dCt2(df, endog_cntrl, cntrl_num = 1, excld_cntrl)

Arguments

df	dataframe: most often the output from tidy_qPCR
endog_cntrl	character: list of character names for endogenous control genes to be averaged
cntrl_num	numeric: integer indicating number of endogenous controls to average
excld_cntrl	character: list of endogenous control genes to exclude if not averaging between multiple

Value

data frame

Examples

qPCR_df <- df %>%
tidy_qPCR() %>%
dCt2()

rhecht95/qPCR documentation built on Dec. 25, 2021, 9:41 a.m.