pines | R Documentation |
An experiment where the effect of genetics and some foliage treatments on the gene expression of pine trees were investigated. There are 4 genetic families 3 treatments in a full factorial design. Then, for each treatment/family combination seeds were sown into 4 boxes, in total 3x4x4 = 48 boxes. These were placed randomly in a growth chamber. After some weeks 5 plants from each box were harvested and gene expression was measured by qPCR.
pines
A data frame with 240 observations (rows) and 4 variables (columns).
Column name | Data type | Description | Values | |
[,1] | ddCt | numeric | Gene expression values | (-17.93 - 8.84) |
[,2] | Treatment | integer | The foliage treatment | (Control, MeJA, PipA) |
[,3] | Family | integer | The genetic families | (a,b,c,d) |
[,4] | Box | integer | The box where the seeds grow, nested under each Treatment/Family combination | (1 - 4) |
These data are not from the real experiment, but from a simulation done before the experiments were conducted, in order to investigate the power of various analyses under various assumptions
# Plot
library(ggplot2)
ggplot(pines, aes(x = Treatment, y = ddCt, color = factor(Box))) +
geom_point(size = 4, alpha = 0.5) +
facet_wrap(~Family, nrow = 2)
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