pines: pines: Experiment with pine trees in growth chambers

pinesR Documentation

pines: Experiment with pine trees in growth chambers

Description

An experiment where the effect of genetics and some foliage treatments on the gene expression of pine trees were investigated. There are 4 genetic families 3 treatments in a full factorial design. Then, for each treatment/family combination seeds were sown into 4 boxes, in total 3x4x4 = 48 boxes. These were placed randomly in a growth chamber. After some weeks 5 plants from each box were harvested and gene expression was measured by qPCR.

Usage

pines

Format

A data frame with 240 observations (rows) and 4 variables (columns).

Column name Data type Description Values
[,1] ddCt numeric Gene expression values (-17.93 - 8.84)
[,2] Treatment integer The foliage treatment (Control, MeJA, PipA)
[,3] Family integer The genetic families (a,b,c,d)
[,4] Box integer The box where the seeds grow, nested under each Treatment/Family combination (1 - 4)

Details

These data are not from the real experiment, but from a simulation done before the experiments were conducted, in order to investigate the power of various analyses under various assumptions

Examples


# Plot
library(ggplot2)
ggplot(pines, aes(x = Treatment, y = ddCt, color = factor(Box))) +
 geom_point(size = 4, alpha = 0.5) +
 facet_wrap(~Family, nrow = 2)


thoree/stat340 documentation built on June 30, 2024, 4:04 p.m.