tobiasgf/lulu: Post Clustering Curation of Amplicon Data for Reliable Biodiversity Metrics

The LULU algorithm excludes artefactual OTUs without discarding rare, but real OTUs. The core mechanism is the identification and merging of ‘daughter’ OTUs with consistently co-occurring, sequence-similar, but more abundant ‘parent’ OTUs across a multi-sample dataset, under the assumption that the ‘daughter’ OTUs are artefacts. The algorithm is independent of a reference database, and can thus be applied to any OTU table produced for any set of samples with any initial OTU definition algorithm.

install.packages("remotes")
remotes::install_github("tobiasgf/lulu")