Uses the external cutadapt progrom to trim off primer sequences
1 2 | clip_primers(fwd_files, rev_files, fwd_primer, rev_primer, out_dir,
cutadapt_bin, samples = NULL, max_n = 0, q = 20, min_len = 50)
|
fwd_files |
list of file names of the forward reads |
rev_files |
list of file names of the reverse reads |
fwd_primer |
forward primer sequnce as a string |
rev_primer |
reverse primer sequnce as a string |
out_dir |
path to directory to write the clipped files |
cutadapt_bin |
location of the cutadapt binary. If you're not sure, run
|
samples |
character vector of samples names, if not provided the the forward file names will be used |
max_n |
discard reads with more than this many Ns |
q |
quality score for 3' end-trimming |
min_len |
minimum length of sequence to retain |
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