This R package provides a function fluorescence.selection
to analyze
fluorescence enriched data.
The package can be installed from github by devtools
install.packages("devtools")
devtools::install_github("wolfsonliu/FluorescenceSelection")
result <- fluorescence.selection(
gene, sgrna, barcode,
reference, fluorescence,
selection.ratio=0.05, multiplier=NA, n.to.consider=NA
)
gene
, sgrna
, barcode
are character vectors of the library sgRNAs
(with barcode). The reference
and fluorescence
are raw counts of
the library, with the same length and order as gene
, sgrna
, and
barcode
.
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