README.md

FluorescenceSelection

This R package provides a function fluorescence.selection to analyze fluorescence enriched data.

Installation

The package can be installed from github by devtools

install.packages("devtools")

devtools::install_github("wolfsonliu/FluorescenceSelection")

Usage

result <- fluorescence.selection(
    gene, sgrna, barcode,
    reference, fluorescence,
    selection.ratio=0.05, multiplier=NA, n.to.consider=NA
)

gene, sgrna, barcode are character vectors of the library sgRNAs (with barcode). The reference and fluorescence are raw counts of the library, with the same length and order as gene, sgrna, and barcode.



wolfsonliu/FluorescenceSelection documentation built on Sept. 14, 2020, 9:01 p.m.