plotTree: Plotting tree information enriched with information

plotTreeR Documentation

Plotting tree information enriched with information

Description

Based on the stored data in a TreeSummarizedExperiment a tree can be plotted. From the rowData, the assays as well as the colData information can be taken for enriching the tree plots with additional information.

Usage

plotRowTree(x, ...)

plotColTree(x, ...)

## S4 method for signature 'TreeSummarizedExperiment'
plotColTree(
  x,
  tree.name = tree_name,
  tree_name = "phylo",
  relabel.tree = relabel_tree,
  relabel_tree = FALSE,
  order.tree = order_tree,
  order_tree = FALSE,
  levels.rm = remove_levels,
  remove_levels = FALSE,
  show.label = show_label,
  show_label = FALSE,
  show.highlights = show_highlights,
  show_highlights = FALSE,
  show.highlight.label = show_highlight_label,
  show_highlight_label = FALSE,
  abbr.label = abbr_label,
  abbr_label = FALSE,
  add.legend = add_legend,
  add_legend = TRUE,
  layout = "circular",
  edge.colour.by = edge.colour.by,
  edge_colour_by = NULL,
  edge.size.by = edge_size_by,
  edge_size_by = NULL,
  tip.colour.by = tip_colour_by,
  tip_colour_by = NULL,
  tip.shape.by = tip_shape_by,
  tip_shape_by = NULL,
  tip.size.by = tip_size_by,
  tip_size_by = NULL,
  node.colour.by = node_colour_by,
  node_colour_by = NULL,
  node.shape.by = node_shape_by,
  node_shape_by = NULL,
  node.size.by = node_size_by,
  node_size_by = NULL,
  colour.highlights.by = colour_highlights_by,
  colour_highlights_by = NULL,
  assay.type = by_exprs_values,
  by_exprs_values = "counts",
  other.fields = other_fields,
  other_fields = list(),
  ...
)

## S4 method for signature 'TreeSummarizedExperiment'
plotRowTree(
  x,
  tree.name = tree_name,
  tree_name = "phylo",
  relabel.tree = relabel_tree,
  relabel_tree = FALSE,
  order.tree = order_tree,
  order_tree = FALSE,
  levels.rm = remove_levels,
  remove_levels = FALSE,
  show.label = show_label,
  show_label = FALSE,
  show.highlights = show_highlights,
  show_highlights = FALSE,
  show.highlight.label = show_highlight_label,
  show_highlight_label = FALSE,
  abbr.label = abbr_label,
  abbr_label = FALSE,
  add.legend = add_legend,
  add_legend = TRUE,
  layout = "circular",
  edge.colour.by = edge_colour_by,
  edge_colour_by = NULL,
  edge.size.by = edge_size_by,
  edge_size_by = NULL,
  tip.colour.by = tip_colour_by,
  tip_colour_by = NULL,
  tip.shape.by = tip_shape_by,
  tip_shape_by = NULL,
  tip.size.by = tip_size_by,
  tip_size_by = NULL,
  node.colour.by = node_colour_by,
  node_colour_by = NULL,
  node.shape.by = node_shape_by,
  node_shape_by = NULL,
  node.size.by = node_size_by,
  node_size_by = NULL,
  colour.highlights.by = colour_highlights_by,
  colour_highlights_by = NULL,
  assay.type = by_exprs_values,
  by_exprs_values = "counts",
  other.fields = other_fields,
  other_fields = list(),
  ...
)

Arguments

x

a TreeSummarizedExperiment x.

...

additional arguments for plotting. See mia-plot-args for more details i.e. call help("mia-plot-args")

tree.name

Character scalar. Specifies a rowTree/colTree from x. (Default: tree.name = "phylo")

tree_name

Deprecated. Use tree.name instead.

relabel.tree

Logical scalar. Should the tip labels be relabeled using the output of getTaxonomyLabels(x, with_rank = TRUE)? (Default: FALSE)

relabel_tree

Deprecated. Use relavel.tree instead.

order.tree

Logical scalar. Should the tree be ordered based on alphabetic order of taxonomic levels? (Default: FALSE)

order_tree

Deprecated. Use order.tree instead.

levels.rm

Logical scalar. Should taxonomic level information be removed from labels? (Default: FALSE)

remove_levels

Deprecated. Use levels.rm instead.

show.label, show.highlights, show.highlight.label, abbr.label

logical (scalar), integer or character vector. If a logical scalar is given, should tip labels be plotted or if a logical vector is provided, which labels should be shown? If an integer or character vector is provided, it will be converted to a logical vector. The integer values must be in the range of 1 and number of nodes, whereas the values of a character vector must match values of the label column in the node data. In case of a character vector only values corresponding to actual labels will be plotted and if no labels are provided no labels will be shown. (default: FALSE)

show_label, show_highlights, show_highlight_label, abbr_label

Deprecated. Use show.label, show.highlights, show.highlight.label, abbr_label instead.

add.legend

Logical scalar. Should legends be plotted? (Default: TRUE)

add_legend

Deprecated. Use add.legend instead.

layout

layout for the plotted tree. See ggtree for details.

edge.colour.by

Character scalar. Specification of a column metadata field or a feature to colour tree edges by, see the by argument in ?retrieveCellInfo for possible values.

edge_colour_by

Deprecated. Use edge.colour.by instead.

edge.size.by

Character scalar. Specification of a column metadata field or a feature to size tree edges by, see the by argument in ?retrieveCellInfo for possible values. (Default: NULL)

edge_size_by

Deprecated. Use edge.size.by instead.

tip.colour.by

Character scalar. Specification of a column metadata field or a feature to colour tree tips by, see the by argument in ?retrieveCellInfo for possible values. (Default: NULL)

tip_colour_by

Deprecated. Use tip.colour.by instead.

tip.shape.by

Character scalar. Specification of a column metadata field or a feature to shape tree tips by, see the by argument in ?retrieveCellInfo for possible values. (Default: NULL)

tip_shape_by

Deprecated. Use tip.shape.by isntead.

tip.size.by

Character scalar. Specification of a column metadata field or a feature to size tree tips by, see the by argument in ?retrieveCellInfo for possible values. (Default: NULL)

tip_size_by

Deprecated. Use tip.size.by instead.

node.colour.by

Character scalar. Specification of a column metadata field or a feature to colour tree nodes by. Must be a field from other.fields. (Default: NULL)

node_colour_by

Deprecated. Use node.colour.by instead.

node.shape.by

Character scalar. Specification of a column metadata field or a feature to shape tree nodes by. Must be a field from other.fields. (Default: NULL)

node_shape_by

Deprecated. Use node.shape.by instead.

node.size.by

Character scalar. Specification of a column metadata field or a feature to size tree nodes by. Must be a field from other.fields. (Default: NULL)

node_size_by

Deprecated. Use node.size.by instead.

colour.highlights.by

Logical scalar. Should the highlights be colour differently? If show.highlights = TRUE, colour_highlights will be set to TRUE as default. (Default: FALSE)

colour_highlights_by

Deprecated. Use colour.highlights.by instead.

assay.type

Character scalar. or integer scalar. Specifies which assay to obtain expression values from, for use in point aesthetics - see the exprs_values argument in ?retrieveCellInfo. (Default: "counts")

by_exprs_values

Deprecated. Use assay.type instead.

other.fields

Character vector. Additional fields to include in the node information without plotting them. (Default: list())

other_fields

Deprecated. Use other.fields instead.

Details

If show.label or show.highlight.label have the same length as the number of nodes, the vector will be used to relabel the nodes.

Value

a ggtree plot

See Also

agglomerateByRanks

Examples

library(scater)
library(mia)
# preparation of some data
data(GlobalPatterns)
GlobalPatterns <- agglomerateByRanks(GlobalPatterns)
altExp(GlobalPatterns,"Genus") <- addPerFeatureQC(altExp(GlobalPatterns,"Genus"))
rowData(altExp(GlobalPatterns,"Genus"))$log_mean <-
  log(rowData(altExp(GlobalPatterns,"Genus"))$mean)
rowData(altExp(GlobalPatterns,"Genus"))$detected <-
   rowData(altExp(GlobalPatterns,"Genus"))$detected / 100
top_genus <- getTop(altExp(GlobalPatterns,"Genus"),
                        method="mean",
                        top=100L,
                        assay.type="counts")
#
x <- altExp(GlobalPatterns,"Genus")
plotRowTree(x[rownames(x) %in% top_genus,],
            tip.colour.by = "log_mean",
            tip.size.by = "detected")

# plot with tip labels
plotRowTree(x[rownames(x) %in% top_genus,],
            tip.colour.by = "log_mean",
            tip.size.by = "detected",
            show.label = TRUE)
# plot with selected labels
labels <- c("Genus:Providencia", "Genus:Morganella", "0.961.60")
plotRowTree(x[rownames(x) %in% top_genus,],
            tip.colour.by = "log_mean",
            tip.size.by = "detected",
            show.label = labels,
            layout="rectangular")

# plot with labeled edges
plotRowTree(x[rownames(x) %in% top_genus,],
            edge.colour.by = "Phylum",
            tip.colour.by = "log_mean")
# if edges are sized, colours might disappear depending on plotting device
plotRowTree(x[rownames(x) %in% top_genus,],
            edge.colour.by = "Phylum",
            edge.size.by = "detected",
            tip.colour.by = "log_mean")

# aggregating data over the taxonomic levels for plotting a taxonomic tree
# please note that the original tree of GlobalPatterns is dropped by
# unsplitByRanks
altExps(GlobalPatterns) <- splitByRanks(GlobalPatterns)
top_phyla <- getTop(altExp(GlobalPatterns,"Phylum"),
                        method="mean",
                        top=10L,
                        assay.type="counts")
altExps(GlobalPatterns) <- lapply(altExps(GlobalPatterns), addPerFeatureQC)
altExps(GlobalPatterns) <-
   lapply(altExps(GlobalPatterns),
          function(y){
              rowData(y)$log_mean <- log(rowData(y)$mean)
              rowData(y)$detected <- rowData(y)$detected / 100
              y
          })
x <- unsplitByRanks(GlobalPatterns)
x <- addHierarchyTree(x)

highlights <- c("Phylum:Firmicutes","Phylum:Bacteroidetes",
                "Family:Pseudomonadaceae","Order:Bifidobacteriales")
plotRowTree(x[rowData(x)$Phylum %in% top_phyla,],
            tip.colour.by = "log_mean",
            node.colour.by = "log_mean",
            show.highlights = highlights,
            show.highlight.label = highlights,
            colour.highlights.by = "Phylum")

plotRowTree(x[rowData(x)$Phylum %in% top_phyla,],
            edge.colour.by = "Phylum",
            edge.size.by = "detected",
            tip.colour.by = "log_mean",
            node.colour.by = "log_mean")

microbiome/miaViz documentation built on Nov. 23, 2024, 8:59 p.m.