plotQuality-methods: Methods for Function 'plotQuality' in Package 'EDASeq'

In EDASeq: Exploratory Data Analysis and Normalization for RNA-Seq

Description

plotQuality produces a plot of the quality of the reads.

Methods

signature(x = "BamFileList")

It produces a plot that summarizes the per-base mean quality of the reads of each BAM file in x.

signature(x = "BamFile")

It produces a boxplot of the per-base distribution of the quality scores of the reads in x.

signature(x = "FastqFileList")

It produces a plot that summarizes the per-base mean quality of the reads of each FASTQ file in x.

Details

Since FASTQ files can be very long, it can be very expensive to process a whole file. One way to avoid this, is to consider a subset of the file and then plot the quality of the subset. As long as one assumes that the subset is random, this is a good approximation. The function FastqSampler of ShortRead can be used for this. See its help page for an example.

EDASeq documentation built on Nov. 8, 2020, 8:29 p.m.