test_Maaslin2.R
In Maaslin2: Maaslin2

library(testthat)
library(Maaslin2)

expected_results_run1 <- read.table("expected_results_run1.tsv", header = TRUE, stringsAsFactors=FALSE, sep="\t")
expected_results_run2 <- read.table("expected_results_run2.tsv", header = TRUE, stringsAsFactors=FALSE, sep="\t")

features <- read.table(system.file(package="Maaslin2","extdata","HMP2_taxonomy.tsv"), header = TRUE, row.names = 1, sep="\t")
metadata <- read.table(system.file(package="Maaslin2","extdata","HMP2_metadata.tsv"), header = TRUE, row.names = 1, sep="\t")

# run with dysbiosis as a nested categorical variable
fit_data <- Maaslin2(features, metadata, 'output', transform = "AST",
    fixed_effects = c('diagnosis', 'dysbiosisnonIBD','dysbiosisUC','dysbiosisCD', 'antibiotics', 'age'),
    random_effects = c('site', 'subject'),
    reference = 'diagnosis,nonIBD',
    standardize = FALSE, plot_heatmap = FALSE, plot_scatter = FALSE)
maaslin_results = read.table(file.path("output","significant_results.tsv"), header = TRUE, stringsAsFactors=FALSE)

expect_that(expected_results_run1$metadata[1:50],equals(maaslin_results$metadata[1:50]))
expect_that(expected_results_run1$feature[1:50],equals(maaslin_results$feature[1:50]))
expect_that(round(expected_results_run1$N[1:50],10),equals(round(maaslin_results$N[1:50],10)))
expect_that(expected_results_run1$N.not.0[1:50],equals(maaslin_results$N.not.0[1:50]))
expect_that(round(as.numeric(expected_results_run1$pval.value[1:50]),10),equals(round(as.numeric(maaslin_results$pval.value[1:50]),10)))
expect_that(round(as.numeric(expected_results_run1$qval.value[1:50]),10),equals(round(as.numeric(maaslin_results$qval.value[1:50]),10)))

# run with with dysbiosis as a continous variable
fit_data <- Maaslin2(features, metadata, 'output2', normalization = "NONE", transform = "AST",
    fixed_effects = c('diagnosis', 'dysbiosis', 'antibiotics', 'age'),
    random_effects = c('site', 'subject'),
    reference = 'diagnosis,nonIBD',
    standardize = FALSE, plot_heatmap = FALSE, plot_scatter = FALSE)
maaslin_results = read.table(file.path("output2","significant_results.tsv"), header = TRUE, stringsAsFactors=FALSE)

expect_that(expected_results_run2$metadata[1:50],equals(maaslin_results$metadata[1:50]))
expect_that(expected_results_run2$feature[1:50],equals(maaslin_results$feature[1:50]))
expect_that(round(expected_results_run2$N[1:50],10),equals(round(maaslin_results$N[1:50],10)))
expect_that(expected_results_run2$N.not.0[1:50],equals(maaslin_results$N.not.0[1:50]))
expect_that(round(as.numeric(expected_results_run2$pval.value[1:50]),10),equals(round(as.numeric(maaslin_results$pval.value[1:50]),10)))
expect_that(round(as.numeric(expected_results_run2$qval.value[1:50]),10),equals(round(as.numeric(maaslin_results$qval.value[1:50]),10)))