replaceNAs: Replace NAs with a given value

In NormqPCR: Functions for normalisation of RT-qPCR data

Description

Replace NAs with a given value

Usage

replaceNAs(qPCRBatch, ...)

## S4 method for signature 'qPCRBatch'
replaceNAs(qPCRBatch, newNA)

Arguments

qPCRBatch	Expression set containing qPCR data.
...	Extra arguments, detailed below
newNA	The new value to replace the NAs with

Details

Replaces NA values in the exprs slot of the qPCRBatch object with a given number

Value

qPCRBatch object with a new exprs slot

Author(s)

James Perkins [e-mail]

References

Perkins, JR, Dawes, JM, McMahon, SB, Bennett, DL, Orengo, C, Kohl, M (2012). ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data. BMC Genomics, 13, 1:296.

Examples

  path <- system.file("exData", package = "NormqPCR")
  taqman.example <- file.path(path, "example.txt")
  qPCRBatch.taqman <- read.taqman(taqman.example)
  qPCRBatch.taqman.replaced <- replaceNAs(qPCRBatch.taqman, newNA = 40)
  exprs(qPCRBatch.taqman.replaced)["Ccl20.Rn00570287_m1",]

NormqPCR documentation built on Nov. 8, 2020, 6:37 p.m.