geneStabM: Gene expression stability value M

In SLqPCR: Functions for analysis of real-time quantitative PCR data at SIRS-Lab GmbH

Description

Computation of the gene expression stability value M for real-time quantitativ RT-PCR data. For more details we refer to Vandesompele et al. (2002).

Usage

geneStabM(relData, na.rm = FALSE)

Arguments

relData	matrix or data.frame containing real-time quantitative RT-PCR data
na.rm	a logical value indicating whether NA values should be stripped before the computation proceeds.

Details

The gene expression stability value M is defined as the average pairwise normalization factor; i.e., one needs to specify data from at least two genes. For more details see Vandesompele et al. (2002).

Value

numeric vector with gene expression stability values

Author(s)

Dr. Matthias Kohl (SIRS-Lab GmbH) [e-mail]

References

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002. 3(7):research0034.1-0034.11. http://genomebiology.com/2002/3/7/research/0034/

SLqPCR documentation built on Nov. 8, 2020, 5:12 p.m.