Description Usage Arguments Value Author(s) References
Compute relative expression values for realtime quantitative RT-PCR data based on Ct or take-off values, respectively. The computations use the PCR efficiency.
1 | relQuantPCR(x, E = 2, na.rm = FALSE)
|
x |
numeric vector containing raw data |
E |
PCR efficiency |
na.rm |
a logical value indicating whether |
vector of relative expression values w.r.t. specified PCR efficiency.
Dr. Matthias Kohl (SIRS-Lab GmbH) kohl@sirs-lab.com
Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002. 3(7):research0034.1-0034.11. http://genomebiology.com/2002/3/7/research/0034/
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