small_airway: Small Airway 'SingleCellExperiment' object
In aggregateBioVar: Differential Gene Expression Analysis for Multi-subject scRNA-seq

Small airway epithelium single-cell RNA sequencing data subset combined from 7 porcine individuals. Genotypes represent wildtype (Genotype WT; n=3) and CFTR-knockout subjects (Genotype CFTRKO; n=4) expressing a cystic fibrosis phenotype.

To access cell counts and column metadata:

counts: SummarizedExperiment::assay(small_airway, "counts")
metadata: SummarizedExperiment::colData(small_airway)

Cell types include:

Secretory cell
Endothelial cell
Immune cell

1	small_airway

A SingleCellExperiment object with feature counts of 1311 genes (rows) from 2687 individual cells (columns). Features were subset by gene ontology annotation "ion transport" with included child terms ("anion transport", "cation transport", etc.). Includes a count matrix accessed by assay and column metadata accessed by colData. Metadata variables are defined as:

orig.ident

Biological sample identifier (i.e. subject)

nCount_RNA

Number of UMIs (i.e. depth/size factor)

nFeature_RNA

Features with non-zero counts (i.e. identified features)

Genotype

Sample Genotype ('CF' == cystic fibrosis)