getLibraryPlate: 384-well plate assay format to a 96-well plate library format

In cellHTS: Analysis of cell-based screens

Description

Given a cellHTS object with data from an assay conducted in 384-well plate format, resulting from the combination of four consecutive 96-well plates of a reagent library, this function gives the plate identifiers for the 96-well plates.

Usage

getLibraryPlate(x)

Arguments

x	a cellHTS object.

Details

The cellHTS object x contains data from a screening experiment where every set of four consecutive 96-well plates was combined into a 384-well plate. Therefore, the only available plate identifiers are for the assay plate format (384-well plates). The way the four 96-well plates are transferred to a 384-well plate during an experiment is as follows: the robot stars by transferring the samples from the first 96-well plate into the first quadrant of the 384-well plate, and so on.

Value

An S3 object of class cellHTS, which extends the argument x by the following element:

libPlate

a vector of length equal to the total number of wells of all the 384-well plates, containing a number that identifies the 96-well plate. It ranges from 1 to four times the total number of 384-well plates.

Author(s)

Ligia Braz [e-mail]

Examples

    data(KcViabSmall)
    x <- getLibraryPlate(KcViabSmall)
    table(x$libPlate)

cellHTS documentation built on Oct. 5, 2016, 4:40 a.m.