Many two-colour hybridizations suffer from a dye bias that is both gene-specific and slide-specific. The former depends on the content of the nucleotide used for labeling; the latter depends on the labeling percentage. The slide-dependency was hitherto not recognized, and made addressing the artefact impossible. Given a reasonable number of dye-swapped pairs of hybridizations, or of same vs. same hybridizations, both the gene- and slide-biases can be estimated and corrected using the GASSCO method (Margaritis et al., Mol. Sys. Biol. 5:266 (2009), doi:10.1038/msb.2009.21)
|Author||Philip Lijnzaad and Thanasis Margaritis|
|Bioconductor views||Microarray Preprocessing QualityControl TwoChannel|
|Maintainer||Philip Lijnzaad <email@example.com>|
|Package repository||View on Bioconductor|
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