BeadFlowFrame-class: BeadFlowFrame

In flowBeads: flowBeads: Analysis of flow bead data

Description

Extension of flowFrame specific for bead data.

The constructor take as arguments the FCS file and the file containing the MEF values of the beads on the different detector channels

Usage

  BeadFlowFrame(fcs.filename, bead.filename)

Arguments

fcs.filename	The file name of the FCS to load. File is loaded with the read.FCS function.
bead.filename	The file name of the MEF configuration files indicating the type of beads in the FCS file. The bead.file is read with read.csv.

Slots

fcs.filename:

The file name of the FCS file from which to read.

bead.filename:

The file name of the bead config file.

beads.mef:

The data.frame containing the MEF of the bead populations on different channels.

trans:

The transform f to linearise the fluorescence.

inv.trans:

The inverse transform of f^{-1}.

flowBeads documentation built on Nov. 8, 2020, 5:01 p.m.