proliferationIndex: proliferation index calculator

In flowFit: Estimate proliferation in cell-tracking dye studies

Description

Proliferation index calculator. Proliferation index is calculated as the sum of the cells in all generations including the parental divided by the computed number of original parent cells theoretically present at the start of the experiment. It is a measure of the fold increase in cell number in the culture over the course of the experiment.

Usage

proliferationIndex(object)

Arguments

object

An object of class proliferationFittingData

Details

The formula is: \frac{∑_0^iN_i}{∑_0^iN^i/2^i} Where i is the generation number (parent generation = 0). In the absence of proliferation, that is, when all cells are in the parent generation, the formula gives: \frac{N_0}{N_0/2^0} = 1 defining the lower limit of the PI.

Value

return a numeric

Author(s)

Davide Rambaldi

References

1. Munson ME. An improved technique for calculating relative response in cellular proliferation experiments. Cytometry A. 2010 Oct;77(10):909-10. doi: 10.1002/cyto.a.20935. Erratum in: Cytometry A. 2010 Dec;77(12):1177. PubMed PMID: 21290464.

See Also

proliferationFitting proliferationFittingData-class

Examples

  # load data
  if(require(flowFitExampleData)){
    data(PKH26data)
    parent.fitting <-  parentFitting(PKH26data[[1]], "FL2-Height LOG")
    my.fit <- proliferationFitting(PKH26data[[2]], "FL2-Height LOG", 
                                   parent.fitting@parentPeakPosition, 
                                   parent.fitting@parentPeakSize)
    my.index <- proliferationIndex(my.fit)
  }

Example output

Loading required package: flowFitExampleData
Warning message:
In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE,  :
  there is no package called 'flowFitExampleData'

flowFit documentation built on April 28, 2020, 9:04 p.m.