flowJoCompensate-methods: Compensate flow cytometry data the same way as FlowJo does
In flowFlowJo: Tools for extracting information from a FlowJo workspace and working with the data in the flowCore paradigm.
Description
FlowJo currently implements its compensation/spillover matrices
differently than they are implemented in the general flow cytometry
community. Currently, in order to obtain similar results (e.g. MFIs
and cell counts) between FlowJo and flowCore, it is necessary to apply
the compensation matrix to the data in the usual way (ie. via “compensate”),
and then to divide all of the observed data by the maximum of the
values in the compensation matrix. The flowFlowJo package implements
a method, flowJoCompensate, to automatically take care of this issue.
Methods
- flowData = "flowFrame", compMatrix = "matrix"
-
flowJoCompensate can take in either a flowFrame or a flowSet as well as a numerical matrix of compensation values.
- flowData = "flowSet", compMatrix = "matrix"
-
flowJoCompensate can take in either a flowFrame or a flowSet as well as a numerical matrix of compensation values.
flowFlowJo documentation built on Sept. 12, 2016, 6:14 a.m.
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Description
FlowJo currently implements its compensation/spillover matrices differently than they are implemented in the general flow cytometry community. Currently, in order to obtain similar results (e.g. MFIs and cell counts) between FlowJo and flowCore, it is necessary to apply the compensation matrix to the data in the usual way (ie. via “compensate”), and then to divide all of the observed data by the maximum of the values in the compensation matrix. The flowFlowJo package implements a method, flowJoCompensate, to automatically take care of this issue.
Methods
- flowData = "flowFrame", compMatrix = "matrix"
-
flowJoCompensate can take in either a flowFrame or a flowSet as well as a numerical matrix of compensation values.
- flowData = "flowSet", compMatrix = "matrix"
-
flowJoCompensate can take in either a flowFrame or a flowSet as well as a numerical matrix of compensation values.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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