FlowJo currently implements its compensation/spillover matrices differently than they are implemented in the general flow cytometry community. Currently, in order to obtain similar results (e.g. MFIs and cell counts) between FlowJo and flowCore, it is necessary to apply the compensation matrix to the data in the usual way (ie. via “compensate”), and then to divide all of the observed data by the maximum of the values in the compensation matrix. The flowFlowJo package implements a method, flowJoCompensate, to automatically take care of this issue.
flowJoCompensate can take in either a flowFrame or a flowSet as well as a numerical matrix of compensation values.
flowJoCompensate can take in either a flowFrame or a flowSet as well as a numerical matrix of compensation values.
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