flowSpecs-package: Tools for processing high-dimensional cytometry files
In flowSpecs: Tools for processing of high-dimensional cytometry data
Description
Author(s)
See Also
Description
This package is intended to fill the role of conventional
cytometry pre-processing software, for spectral decomposition,
transformation, visualization and cleanup, and to aid further downstream
analyses, such as with DepecheR, by enabling transformation of flowFrames
and flowSets to dataframes. Functions for flowCore-compliant automatic
1D-gating/filtering are in the pipe line.
It is worth noting here that even if there are dedicated spectral cytometers,
it is possible to increase the separation of the fluorochromes in a
conventional flow cytometer too, by just keeping all non-used channels open.
That will however also require the use of spectral unmixing, rather than
compensation, as the compensation functions generally require the
compensation matrix to be symmetrical. So please open all channels, and use
this software!
Author(s)
Maintainer: Jakob Theorell <jakob.theorell@ndcn.ox.ac.uk>
See Also
flowSpecs documentation built on Nov. 8, 2020, 5:39 p.m.
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Description Author(s) See Also
Description
This package is intended to fill the role of conventional cytometry pre-processing software, for spectral decomposition, transformation, visualization and cleanup, and to aid further downstream analyses, such as with DepecheR, by enabling transformation of flowFrames and flowSets to dataframes. Functions for flowCore-compliant automatic 1D-gating/filtering are in the pipe line. It is worth noting here that even if there are dedicated spectral cytometers, it is possible to increase the separation of the fluorochromes in a conventional flow cytometer too, by just keeping all non-used channels open. That will however also require the use of spectral unmixing, rather than compensation, as the compensation functions generally require the compensation matrix to be symmetrical. So please open all channels, and use this software!
Author(s)
Maintainer: Jakob Theorell <jakob.theorell@ndcn.ox.ac.uk>
See Also
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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