microVS: Variance stabilization for microarray data.

In flowVS: Variance stabilization in flow cytometry (and microarrays)

Description

Variance-stabilizing inverse hyperbolic sine (asinh) transformation for microarray data.

Usage

  microVS(data, cfLow=0, cfHigh=10, frac=1)

Arguments

data	The microarray data in a Matrix.
cfLow	lowest possible value of cofactor (log scale).
cfHigh	highest possible value of cofactor (log scale).
frac	fraction of differentially expressed genes used in variance stabilization (0< frac <=1).

Details

This function transforms a microarray data matrix z by asinh(z/c) transformation where c is a normalizing cofactor. The cofactor is searched in the range [cfLow, cfHigh] and an optimum cofactor is obtained for which the transformed data is variance stabilized. The optimum cofactor is obtained by minimizing Bartlett's test statistics for homogeneity of variance. If the parameter frac is less then one, a fraction of differentially expressed genes are used in estimating the cofactor.

Value

microVS returns a matrix of the variance-stabilizing microarray data.

Author(s)

Ariful Azad

References

Ariful Azad, Bartek Rajwa, and Alex Pothen (2015), "flowVS: Channel-Specific Variance Stabilization in Flow Cytometry", BMC Bioinformatics, vol 17, pp 1-14, 2016.

Examples

# stabilize variance of the Kidney microarray data from the vsn package
library(vsn)
data(kidney)
kidney.t = microVS(exprs(kidney))
plotMeanSd(kidney.t)

flowVS documentation built on April 7, 2021, 6 p.m.