visualizeStereogene: visualizeStereogene

In nearBynding: Discern RNA structure proximal to protein binding

Description

Creates a visual output of a single RNA structure context relative to protein binding.

Usage

visualizeStereogene(
    dir_stereogene_output = ".",
    context_file,
    protein_file,
    protein_file_input = NULL,
    x_lim = c(-100, 100),
    y_lim = NULL,
    out_file = "out_file",
    legend = TRUE,
    heatmap = FALSE
)

Arguments

dir_stereogene_output	Directory of stereogene output. Default working directory.
context_file	A single context file name for visualization with the protein_file(s). File names must exclude extensions such as ".bedGraph". Required.
protein_file	A vector of at least one protein file name to be averaged for visualization. File names must exclude extensions such as ".bedGraph". All files in the list should be experimental or biological replicates. Required.
protein_file_input	A protein file name of background input to be subtracted from protein_file signal. File name must exclude extension. Only one input file is permitted. Optional.
x_lim	A vector of two integers denoting the lower and upper x axis limits. Cannot exceed wSize/2 from write_config. Default (-100, 100)
y_lim	A vector of two numbers denoting the lower and upper y axis limits. Optional.
out_file	Name of output file, excluding extension. ".pdf" or ".jpeg" will be added as relevant to the output file name. Default "out_file"
legend	Whether a legend should be included with the output graph. Default TRUE.
heatmap	Whether the output graph should be in the form of a heatmap (TRUE) or of a line graph (FALSE). Default FALSE

Value

heatmap (JPEG) or line graph (PDF) image file

Examples

## pull example files
get_outfiles()
## heatmap
visualizeStereogene(context_file = "chr4and5_3UTR_stem_liftOver",
                    protein_file = "chr4and5_liftOver",
                    out_file = "stem_heatmap",
                    x_lim = c(-500, 500))
## line graph
visualizeStereogene(context_file = "chr4and5_3UTR_stem_liftOver",
                    protein_file = "chr4and5_liftOver",
                    heatmap = TRUE,
                    out_file = "stem_line",
                    x_lim = c(-500, 500))

nearBynding documentation built on Nov. 8, 2020, 8:15 p.m.