ctlSeparatn: Calculate control separation
In phenoDist: Phenotypic distance measures

Description Usage Arguments Details Value Author(s) References See Also Examples

View source: R/qualityControl.R

This function calculates the Z'-factor between negative and positive controls, based on phenotypic distance measurements.

1	ctlSeparatn(x, pheno, neg='rluc', pos='ubc', ...)

`x`	An `imageHTS` object.
`pheno`	A numeric vector for distances to negative controls, as returned from `distToNeg`.
`neg`	A character string to identify negative controls.
`pos`	A character string to identify positive controls.
`...`	Additional arguments to be passed to the `Zprime` function of the `imageHTS` package.

Phenotypes of negative and positive controls are defined as their phenotypic distance to negative controls. The Z'-factor is a metric measuring the separation between negative controls and positive controls (Zhang et al. 1999). This function calls the Zprime function of the imageHTS package to calculate the Z'-factor. Please see Zprime for detailed description of the definition and calculation of the Z'-factor.

The Z'-factor value.

Xian Zhang

J. H. Zhang, T. D. Chung, K. R. Oldenburg. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screening, 1999.

distToNeg, Zprime