Description Usage Arguments Details Value Author(s) See Also Examples

This function create an an `ExpressionSet`

containing 2D Gel Volume Data and associated metadata. It requires a matrix of 2D Gel Volume data with Gels as columns and Spots as rows as well as a dataframe describing the data.

1 |

`data` |
a matrix of spots intensities. |

`n1` |
an integer. Number of replicates in condition 1. |

`n2` |
an integer. Number of replicates in condition 2. |

`f` |
a dataframe giving the factors for data. |

Usually `data`

matrix should be the output of `Norm.qt`

and therefore is log2-transformed. In `data`

the columns (i.e. Gels) must be ordered with replicates from condition 1 before those from condition 2.

The function returns an `ExpressionSet`

. The matrix of spots intensities (i.e. Volume) is stored in the `assayData`

slot of the `ExpressionSet`

and can be retrieved with `exprs`

. In the `featureData`

slot the log2-ratio is computed and can be retrieved with `fData`

.

Sebastien Artigaud sebastien.artigaud@gmx.com

`Norm.qt`

,`Norm.vsn`

,`ExpressionSet`

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