vsDEGMatrix: Differential gene expression matrix

Description Usage Arguments Value Author(s) Examples

View source: R/vsDEGmat.R

Description

This function allows you to visualize the number of differentially expressed genes (DEG) at a given adjusted P-value for each experimental treatment level. Higher color intensity correlates to a higher number of DEGs.

Usage

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vsDEGMatrix(data, padj = 0.05, d.factor = NULL, type = c("cuffdiff",
  "deseq", "edger"), title = TRUE, legend = TRUE, grid = TRUE,
  data.return = FALSE, grey.scale = FALSE, xaxis.text.size = 10,
  yaxis.text.size = 10, main.title.size = 15, legend.text.size = 10,
  legend.title.size = 12)

Arguments

data

output generated from calling the main routines of either cuffdiff, DESeq2, or edgeR analyses. For cuffdiff, this will be a *_exp.diff file. For DESeq2, this will be a generated object of class DESeqDataSet. For edgeR, this will be a generated object of class DGEList.

padj

a user defined adjusted p-value cutoff point. Defaults to 0.05.

d.factor

a specified factor; for use with DESeq2 objects only. This input equates to the first parameter for the contrast argument when invoking the results() function in DESeq2. Defaults to NULL

type

an analysis classifier to tell the function how to process the data. Must be either cuffdiff, deseq, or edger. cuffdiff must be used with cuffdiff data; deseq must be used for DESeq2 output; edgeR must be used with edgeR data. See the data parameter for further details.

title

display the main title of plot. Logical; defaults to TRUE. If set to FALSE, no title will display in plot.

legend

display legend of plot. Logical; defaults to TRUE. If set to FALSE, no legend will display in plot.

grid

display major and minor axis lines. Logical; defaults to TRUE. If set to FALSE, no axis lines will display in plot.

data.return

returns data output of plot. Logical; defaults to FALSE. If set to TRUE, a data frame will also be called. Assign to object for reproduction and saving of data frame. See final example for further details.

grey.scale

displays grey color scheme instead of blue if set to TRUE. Logical; defaults to FALSE.

xaxis.text.size

change the font size of the x-axis text. Defaults to 10.

yaxis.text.size

change the font size of the y-axis text. Defaults to 10.

main.title.size

change the font size of the plot title text. Defaults to 15.

legend.text.size

change the font size of the legend body text. Defaults to 10.

legend.title.size

change the font size of the legend title text. Defaults to 12.

Value

An object created by ggplot

Author(s)

Brandon Monier, brandon.monier@sdstate.edu

Examples

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# cuffdiff example
data("df.cuff")
vsDEGMatrix(
     df.cuff, padj = 0.05, d.factor = NULL, type = "cuffdiff",
     title = TRUE, legend = TRUE, grid = TRUE
)

# DESeq2 example
data("df.deseq")
require(DESeq2)
vsDEGMatrix(
     df.deseq, padj = 0.05, d.factor = "condition", type = "deseq",
     title = TRUE, legend = TRUE, grid = TRUE
)

# edgeR example
data("df.edger")
require(edgeR)
vsDEGMatrix(
     df.edger, padj = 0.05, d.factor = NULL, type = "edger",
     title = TRUE, legend = TRUE, grid = TRUE
)

# Extract data frame from visualization
data("df.edger")
require(edgeR)
tmp <- vsDEGMatrix(
     df.edger, padj = 0.05, d.factor = NULL, type = "edger",
     title = TRUE, legend = TRUE, grid = TRUE
)
df_deg <- tmp[[1]] ## or use tmp$data
head(df_deg)

# Show plot from object (see prior example for more details)
tmp[[2]] ## or use tmp$plot

vidger documentation built on Nov. 8, 2020, 5:27 p.m.