cyt_ttest: Two Sample T-test Comparisons.

In CytoProfile: Cytokine Profiling Analysis Tool

Two Sample T-test Comparisons.

Description

This function performs pairwise comparisons between two groups for each combination of a categorical predictor (with exactly two levels) and a continuous outcome variable. It first converts any character variables in data to factors and, if specified, applies a log2 transformation to the continuous variables. Depending on the value of scale, the function conducts either a two-sample t-test (if scale = "log2") or a Mann-Whitney U test (if scale is NULL). The resulting p-values are printed and returned.

Usage

cyt_ttest(data, scale = NULL, verbose = TRUE, format_output = FALSE)

Arguments

data	A matrix or data frame containing continuous and categorical variables.
scale	A character specifying a transformation for continuous variables. Options are NULL (default) and "log2". When scale = "log2", a log2 transformation is applied and a two-sample t-test is used; when scale is NULL, a Mann-Whitney U test is performed.
verbose	A logical indicating whether to print the p-values of the statistical tests. Default is TRUE.
format_output	Logical. If TRUE, returns the results as a tidy data frame. Default is FALSE.

Value

If format_output is FALSE, returns a list of p-values (named by Outcome and Categorical variable). If TRUE, returns a data frame in a tidy format.

Examples

data_df <- ExampleData1[, -c(3)]
data_df <- dplyr::filter(data_df, Group != "ND", Treatment != "Unstimulated")
# Two sample T-test with log2 transformation
cyt_ttest(data_df[, c(1, 2, 5:6)], scale = "log2", verbose = TRUE, format_output = TRUE)
# Mann-Whitney U Test without transformation
cyt_ttest(data_df[, c(1, 2, 5:6)], verbose = TRUE, format_output = FALSE)

CytoProfile documentation built on April 11, 2025, 6:10 p.m.