R/V2.R

In FCSlib: A Collection of Fluorescence Fluctuation Spectroscopy Analysis Methods

#' Line-scan data of HEK-293 cells expressing Venus (EYFP) dimers
#'
#' This data set consists on a raster line scan performed over HEK-293 cells expressing dimers of the fluorescent protein Venus, also known as SEYFP-F46L.
#' The scan line is 64 pixels long, and the scanning direction is from the cytoplasm to the nucleus, across the nuclear envelope.
#' A pixel size of 50 nm was used, as well as a pixel dwell time of 12.5 us and a line scan time of 1.925 ms.
#' Fluorescence excitation was provided by a 488 nm laser at 0.1% power.
#' Fluorescence intensity data was collected using the photon-counting mode in an Olympus FV1000 Upright BX61WI confocal microscope.
#'
#' @docType data
#' @keywords datasets
#' @name V2
#' @usage data(V2)
#' @format A matrix with 64 rows and 25000 columns
#' @details To analyze this data set with FCSlib, import by typing: readFileTiff("V2.tif")[,,1]
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