anctrain: Construction of prototype alleles

In GHap: Genome-Wide Haplotyping

Construction of prototype alleles

Description

This function builds prototype alleles to be used in ancestry predictions.

Usage

 ghap.anctrain(object, train = NULL,
               method = "unsupervised",
               K = 2, iter.max = 10, nstart = 10,
               nmarkers = 5000, tune = FALSE,
               only.active.samples = TRUE,
               only.active.markers = TRUE,
               batchsize = NULL, ncores = 1,
               verbose = TRUE)

Arguments

The following arguments are used by both the 'supervised' and 'unsupervised' methods:

object	A GHap.phase object.
train	Character vector of individuals to use as reference samples. All active individuals are used if this vector is not provided.
method	Character value indicating which method to use: 'supervised' or 'unsupervised' (default).
only.active.samples	A logical value specifying whether only active samples should be included in predictions (default = TRUE).
only.active.markers	A logical value specifying whether only active markers should be used for predictions (default = TRUE).
batchsize	A numeric value controlling the number of markers to be processed at a time (default = nmarkers/10).
ncores	A numeric value specifying the number of cores to be used in parallel computing (default = 1).
verbose	A logical value specfying whether log messages should be printed (default = TRUE).

The following arguments are only used by the 'unsupervised' method:

K	A numeric value specifying the number of clusters in K-means (default = 2). Proxy for the number of ancestral populations.
iter.max	A numeric value specifying the maximum number of iterations of the K-means clustering (default = 10).
nstart	A numeric value specifying the number of independent runs of the K-means clustering (default = 10).
nmarkers	A numeric value specifying the number of seeding markers to be used by the K-means clustering (default = 10).
tune	A logical value specfying if a Best K analysis should be performed (default = FALSE).

Details

This function builds prototype alleles (i.e., cluster centroids, representing lineage-specific allele frequencies) through two methods:

The 'unsupervised' method uses the K-means clustering algorithm to group haplotypes into K pseudo-lineages. A random sample of seeding markers (default value of nmarkers = 5000) is used to group all 2*nsamples haplotypes in a user-specified number of clusters (default value of K = 2). Then, for each interrogated block, prototype alleles are built for every cluster using the arithmetic mean of observed haplotypes initially assigned to that cluster. If train = NULL, the function uses all active haplotypes to build prototype alleles. If the user is working with a severely unbalanced data set (ex. one population with a large number of individuals and others with few individuals), it is recommended that a vector of individual names is provided via the train argument such that prototype alleles are built using a more balanced subset of the data.

The 'supervised' method works in a similar way, but skips the K-means algorithm and uses population labels present in the GHap.phase object as clusters.

Value

The function returns a dataframe with the first column giving marker names and remaining columns containing prototype alleles for each pseudo-lineage. If method 'unsupervised' is ran with tune = TRUE, the function returns the following list:

ssq	Within-cluster sum of squares for each value of K.
chindex	Calinski Harabasz Index for consecutive values of K.
pchange	Percent change in ssq for consecutive values of K.

Author(s)

Yuri Tani Utsunomiya <[e-mail]>

References

Y.T. Utsunomiya et al. Unsupervised detection of ancestry tracks with the GHap R package. Methods in Ecology and Evolution. 2020. 11:1448–54.

See Also

ghap.anctest, ghap.ancsmooth, ghap.ancplot, ghap.ancmark

Examples

# #### DO NOT RUN IF NOT NECESSARY ###
# 
# # Copy phase data in the current working directory
# exfiles <- ghap.makefile(dataset = "example",
#                          format = "phase",
#                          verbose = TRUE)
# file.copy(from = exfiles, to = "./")
# 
# # Load phase data
# 
# phase <- ghap.loadphase("example")
# 
# ### RUN ###
# 
# # Calculate marker density
# mrkdist <- diff(phase$bp)
# mrkdist <- mrkdist[which(mrkdist > 0)]
# density <- mean(mrkdist)
# 
# # Generate blocks for admixture events up to g = 10 generations in the past
# # Assuming mean block size in Morgans of 1/(2*g)
# # Approximating 1 Morgan ~ 100 Mbp
# g <- 10
# window <- (100e+6)/(2*g)
# window <- ceiling(window/density)
# step <- ceiling(window/4)
# blocks <- ghap.blockgen(phase, windowsize = window,
#                         slide = step, unit = "marker")
# 
# # BestK analysis
# bestK <- ghap.anctrain(object = phase, K = 5, tune = TRUE)
# plot(bestK$ssq, type = "b", xlab = "K",
#      ylab = "Within-cluster sum of squares")
# 
# # Unsupervised analysis with best K
# prototypes <- ghap.anctrain(object = phase, K = 2)
# hapadmix <- ghap.anctest(object = phase,
#                          blocks = blocks,
#                          prototypes = prototypes,
#                          test = unique(phase$id))
# anctracks <- ghap.ancsmooth(object = phase, admix = hapadmix)
# ghap.ancplot(ancsmooth = anctracks)
# 
# # Supervised analysis
# train <- unique(phase$id[which(phase$pop != "Cross")])
# prototypes <- ghap.anctrain(object = phase, train = train,
#                             method = "supervised")
# hapadmix <- ghap.anctest(object = phase,
#                          blocks = blocks,
#                          prototypes = prototypes,
#                          test = unique(phase$id))
# anctracks <- ghap.ancsmooth(object = phase, admix = hapadmix)
# ghap.ancplot(ancsmooth = anctracks)

GHap documentation built on July 2, 2022, 1:07 a.m.