Inflect: This function analyzes raw abundance data from a Thermal...

Description Usage Arguments Value

View source: R/Inflect.R

Description

This function analyzes raw abundance data from a Thermal Proteome Profiling experiment and calculates melt temperatures and melt shifts for each protein in the experiment.

Usage

1
Inflect(directory, Temperature, Rsq, NumSD, NReps)

Arguments

directory

the directory where the source data files to be analyzed are saved. This is also the location where the results will be saved.

Temperature

the temperatures from the heat treatment procedure. An example entry Temperature<-c(25,35,39.3,50.1,55.2,60.7,74.9,90)

Rsq

the cutoff to be used for the melt shift curve fit. An example entry would be 0.95

NumSD

the standard deviation cutoff to be used for the calculated melt shifts. For example, if NumSD = 2, proteins with melt shifts greater than 2 standard deviations from the mean will be considered significant.

NReps

the number of replicate experiments to be analyzed

Value

xlsx files with calculated melt shift for each protein in the experiment along with Upset plots that show the overlap in number of proteins stabilized and destabilized between each replicate


Inflect documentation built on March 17, 2021, 1:08 a.m.