Description Usage Arguments Value
This function analyzes raw abundance data from a Thermal Proteome Profiling experiment and calculates melt temperatures and melt shifts for each protein in the experiment.
1 | Inflect(directory, Temperature, Rsq, NumSD, NReps)
|
directory |
the directory where the source data files to be analyzed are saved. This is also the location where the results will be saved. |
Temperature |
the temperatures from the heat treatment procedure. An example entry Temperature<-c(25,35,39.3,50.1,55.2,60.7,74.9,90) |
Rsq |
the cutoff to be used for the melt shift curve fit. An example entry would be 0.95 |
NumSD |
the standard deviation cutoff to be used for the calculated melt shifts. For example, if NumSD = 2, proteins with melt shifts greater than 2 standard deviations from the mean will be considered significant. |
NReps |
the number of replicate experiments to be analyzed |
xlsx files with calculated melt shift for each protein in the experiment along with Upset plots that show the overlap in number of proteins stabilized and destabilized between each replicate
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