InflectWorkflow: This function analyzes raw abundance data from a Thermal...

In Inflect: Melt Curve Fitting and Melt Shift Analysis

Description

This function analyzes raw abundance data from a Thermal Proteome Profiling experiment and calculates melt temperatures and melt shifts for each protein in the experiment.

Usage

InflectWorkflow(Rsq, NumSD, Temperature, Rep, SourcePath, OutputPath)

Arguments

Rsq	the cutoff to be used for the melt shift curve fit. An example entry would be 0.95
NumSD	the standard deviation cutoff to be used for the calculated melt shifts. For example, if NumSD = 2, proteins with melt shifts greater than 2 standard deviations from the mean will be considered significant.
Temperature	the temperatures from the heat treatment procedure. An example entry Temperature<-c(25,35,39.3,50.1,55.2,60.7,74.9,90)
Rep	the number of replicate experiments to be analyzed
SourcePath	The path for the source data
OutputPath	The path for the output data

Value

xlsx files with calculated melt shift for each protein in the experiment

Inflect documentation built on March 17, 2021, 1:08 a.m.