Correction: This function corrects the normalized abundance of each...

In InflectSSP: Melt Curve Fitting and Melt Shift Analysis

This function corrects the normalized abundance of each protein using a correction constant that is calculated in this function. The correction constant is determined using the difference between actual and predicted fit at the proteome level.

Description

This function corrects the normalized abundance of each protein using a correction constant that is calculated in this function. The correction constant is determined using the difference between actual and predicted fit at the proteome level.

Usage

Correction(PSM, UP, Data_CurveFit1Parameters, Data_Normalized, Data_Quantified)

Arguments

PSM	the number of peptide spectrum matches that are deemed acceptable for reporting
UP	the number of unique peptides for a protein that are deemed acceptable for reporting
Data_CurveFit1Parameters	the parameters determined from Curve Fit 1 operation for proteome melts
Data_Normalized	the normalized abundance data for each protein determined in the Normalize function.
Data_Quantified	the median normalized abundance data at the proteome level

Value

the corrected and normalized abundance data for each protein

Examples

## Not run: 
Data_Corrected<-Correction(PSM,UP,Data_CurveFit1Parameters,
Data_Normalized,Data_Quantified)

## End(Not run)

InflectSSP documentation built on April 19, 2023, 9:06 a.m.