InflectSSP: This function is the primary function that calls other...

In InflectSSP: Melt Curve Fitting and Melt Shift Analysis

This function is the primary function that calls other functions in the program.

Description

This function is the primary function that calls other functions in the program.

Usage

InflectSSP(
  Directory,
  NControl,
  NCondition,
  PSM,
  UP,
  CurveRsq,
  PValMelt,
  PValMeltFDR,
  MeltLimit,
  RunSTRING,
  STRINGScore,
  Species
)

Arguments

Directory	the directory where the source data files to be analyzed are saved. This is also the location where the results will be saved.
NControl	the number of Control replicate experiments that are to be analyzed
NCondition	the number of Condition replicate experiments that are to be analyzed
PSM	the number of peptide spectrum matches that are deemed acceptable for reporting
UP	the number of unique peptides for a protein that are deemed acceptable for reporting
CurveRsq	Coefficient of determination criteria for melt curves
PValMelt	p-value criteria for melt shifts
PValMeltFDR	Whether or not the FDR correction for pvalue is used in designation of melts of interest
MeltLimit	the melt shift temperature limit used for determining which proteins to report as significant
RunSTRING	whether or not the STRING function will be run or not in the analysis
STRINGScore	the score to be used in the STRING analysis
Species	species number for bioinformatics search

Value

the proteins that have significant melt shifts from an experiment

Examples

## Not run: 
     Directory<-'/Users/Einstein'
     NControl<-2
     NCondition<-3
     PSM<-2
     UP<-3
     CurveRsq<-.95
     PValMelt<-0.05
     PValMeltFDR<-"No"
     MeltLimit<-3
     RunSTRING<-"Yes"
     STRINGScore<-0.99
     Species<-9606
     InflectSSP(Directory,NControl,
     NCondition,PSM,UP,CurveRsq,PValMelt,PValMeltFDR,
     MeltLimit,RunSTRING,STRINGScore,
     Species)
     
## End(Not run)

InflectSSP documentation built on April 19, 2023, 9:06 a.m.