MultiMelt: Surface melting curve data from direct hybridization...

In MBmca: Nucleic Acid Melting Curve Analysis

Description

A melting curve experiment with twelve microbead populations and the short oligonucleotide capture probe and detection probe for human HRPT1 (hyperparathyroidism 1) and MLC-2v (myosin regulatory light chain 2, ventricular/cardiac muscle isoform).

Format

A data frame with the melting curves of two different capture and detection probe pairs for HRPT1 and MLC-2v. First column contains the temperature (in degree Celsius, 1 degree Celsius per step) followed by melting curves of HRPT1 on twelve microbead populations and melting curves of MLC-2v on twelve microbead populations.

T

a numeric vector for the temperature in degree Celsius

HPRT1.1

a numeric vector, as HPRT1.1 of detection/capture probe HPRT1/HPRT1-cap on microbead population 1

HPRT1.2

a numeric vector, as HPRT1.2 on microbead population 2

HPRT1.3

a numeric vector, as HPRT1.3 on microbead population 3

HPRT1.4

a numeric vector, as HPRT1.4 on microbead population 4

HPRT1.5

a numeric vector, as HPRT1.5 on microbead population 5

HPRT1.6

a numeric vector, as HPRT1.6 on microbead population 6

HPRT1.7

a numeric vector, as HPRT1.7 on microbead population 7

HPRT1.8

a numeric vector, as HPRT1.8 on microbead population 8

HPRT1.9

a numeric vector, as HPRT1.9 on microbead population 9

HPRT1.10

a numeric vector, as HPRT1.10 on microbead population 10

HPRT1.11

a numeric vector, as HPRT1.11 on microbead population 11

HPRT1.12

a numeric vector, as HPRT1.12 on microbead population 12

MLC2v1

a numeric vector, as MLC2v1 of detection/capture probe MLC-2v/MLC-2v-cap on microbead population 1

MLC2v2

a numeric vector, as MLC2v2 on microbead population 2

MLC2v3

a numeric vector, as MLC2v3 on microbead population 3

MLC2v4

a numeric vector, as MLC2v4 on microbead population 4

MLC2v5

a numeric vector, as MLC2v5 on microbead population 5

MLC2v6

a numeric vector, as MLC2v6 on microbead population 6

MLC2v7

a numeric vector, as MLC2v7 on microbead population 7

MLC2v8

a numeric vector, as MLC2v8 on microbead population 8

MLC2v9

a numeric vector, as MLC2v9 on microbead population 9

MLC2v10

a numeric vector, as MLC2v10 on microbead population 10

MLC2v11

a numeric vector, as MLC2v11 on microbead population 11

MLC2v12

a numeric vector, as MLC2v12 on microbead population 12

Details

The melting curve was conducted with short oligonucleotide probes on the surface of microbeads using the VideoScan platform according to Roediger et al. (2012). The dyes and quencher used were Atto 647N and BHQ2.

Source

Data were measured with the VideoScan platform:

A Highly Versatile Microscope Imaging Technology Platform for the Multiplex Real-Time Detection of Biomolecules and Autoimmune Antibodies. S. Roediger, P. Schierack, A. Boehm, J. Nitschke, I. Berger, U. Froemmel, C. Schmidt, M. Ruhland, I. Schimke, D. Roggenbuck, W. Lehmann and C. Schroeder. Advances in Biochemical Bioengineering/Biotechnology. 133:35–74, 2013. https://pubmed.ncbi.nlm.nih.gov/22437246/

Surface Melting Curve Analysis with R. S. Roediger, A. Boehm and I. Schimke. The R Journal. 5(2):37–52, 2013, 2013.

See Also

MFIerror, mcaSmoother, diffQ, diffQ2, DMP, DualHyb

Examples

data(MultiMelt)

MBmca documentation built on Sept. 5, 2021, 5:38 p.m.