Description Usage Format Source References Examples
Labelled neutrophils were adoptively transfered and intravitally imaged (using two-photon microscopy) inside the lung of the recipient mouse. These cells display a fairly directed kind of motion, as they move towards infection foci.
1 | data("Neutrophils")
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An S3 object of class "tracks"; a list with 10 elements. Each element name identifies a cell track. Each element is a matrix containing the following four columns.
t
the time (in seconds)
x
The X coordinate (in micrometers)
y
The Y coordinate (in micrometers)
z
The Z coordinate (in micrometers)
Data were generated in 2012 in the Mark J. Miller Lab, Department of Medicine, Washington University in St Louis, USA.
Zinselmeyer BH, Dempster J, Wokosin DL, Cannon JJ, Pless R, Parker I and Miller MJ (2009), Two-photon microscopy and multi-dimensional analysis of cell dynamics. Methods in Enzymology, 461:349–78. doi:10.1016/S0076-6879(09)05416-0
Konjufca V and Miller MJ (2009), Imaging Listeria monocytogenes infection in vivo. Current Topics in Microbiology and Immunology, 334:199–226. doi:10.1007/978-3-540-93864-4_9
Kreisel D, Nava RG, Li W, Zinselmeyer BH, Wang B, Lai J, Pless R, Gelman AE, Krupnick AS, and Miller MJ (2010), In vivo two-photon imaging reveals monocyte-dependent neutrophil extravasation during pulmonary inflammation. PNAS, 107(42):18073–18078. doi:10.1073/pnas.1008737107
1 2 3 4 | ## load the tracks
data(Neutrophils)
## visualize the tracks (calls function plot.tracks)
plot(Neutrophils)
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