Description Usage Arguments Value References See Also Examples
The itcONE11
function calculates the heat exchange for each ITC injection with a given reaction system and reaction parameters as input. Here a given reaction system should be two binding partners with known binding affinity and binding enthalpy. The two binding partners should bind to each other according to the one-to-one binding model, although the binding stoichiometry (N) can be adjusted to account for partial inactivity of one binding partner.
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varpar |
A list of four parameters: K, The binding constant, in 1/M, as in K=[AB]/[A]/[B]; H, The binding enthalpy, in calories; HD, The dilution heat for each injection; N, The stoichiometry, or the fraction of protein being active in ITC cell; |
stapar |
A list of four parameters: P0, The initial protein concentration in ITC cell, in mM; L0, The initial ligand concentration in ITC cell, in mM; Asyr, The initial ligand concentration in syringe, in mM; V0, The volume of ITC cell, in ml; |
injV0 |
A vector of injection volumes for each ITC injection, in ul |
A vector of heat exchange per mole of injectant for each injection, in cal/mole
Freire, E. 1998 Statistical thermodynamic linkage between conformational and binding equilibria. Adv. Protein Chem 51, 255-279.
Tellinghuisen, J. 2007 Calibration in isothermal titration calorimetry: heat and cell volume from heat of dilution of NaCl(aq). Anal. Biochem 360, 47-55.
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