Description Usage Arguments Details Value Author(s) References Examples
Calculation of common root system architecture (RSA) traits from Data Analysis of Root Tracings (DART) output files and RSA data encoded with the Root System Markup Language (RSML). More information can be found in Delory et al (2016), Le Bot et al (2010), and Lobet et al (2015).
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inputrac 
A character string specifying the path to the folder containing the rac files created by DART. This argument could be the same as 
inputtps 
A character string specifying the path to the folder containing the tps files created by DART. This argument could be the same as 
inputrsml 
A character string specifying the path to the folder containing the RSML files. This argument could be the same as 
res 
Mandatory for DART files only. If images were acquired with a flatbed scanner: a numeric value specifying the resolution of the images used to vectorize the root systems with DART (resolution is expressed in dots/inch). If images were acquired with a digital camera: the ratio between the length of a reference object located on the image expressed in pixels and the actual length of the same object expressed in inches. For DART files, this argument must be specified if 
unitlength 
A character string specifying the unit of length that must be used to express the results. The value acceptable for this argument could be either “px” for pixels, “cm” for centimetres or “mm” for millimetres. 
rsml.date 
Only used for RSML files. A numeric value specifying the root system age (the unit of time is defined by the user). If 
rsml.connect 
Only used for RSML files. A logical value that must be specified when 
To run architect
efficiently, DART (.rac or .tps) and RSML (.rsml) files must have been saved with their appropriate extension.
If inputtps
contains a single tps file, it is used by architect
for each rac file located in inputrac
. If inputtps
contains more than one tps file, the number of tps files in inputtps
must be equal to the number of rac files in inputrac
and corresponding rac and tps files must have the same name.
After reading the RSML files located in inputrsml
, the data associated with each root system are converted into a data frame possessing the same structure as the rac files created by DART. The architect
function then uses these data frames to calculate RSA traits describing the global architecture of each root system.
The argument rsml.date
is used to create a tps file for each root system located in inputrsml
. If the root system age is stored as a continuous function along the root segments, the rsml.date
argument can be a character string specifying the name/label of the function. For instance, the root system age at the first apparition of each node is stored as a continuous function called “age” in RSML files containing RSA data simulated with the ArchiSimple model (Pages et al (2013)). The “age” function attaches the root system age along the root segments, so that each node is associated with one age value. An RSML library containing 3D RSA data simulated with ArchiSimple is available here: https://doi.org/10.5281/zenodo.208214.
The rsml.connect
argument can be used to connect the lateral roots to their corresponding mother root. If rsml.connect=TRUE
, each point starting a lateral root is connected to the nearest point located on its mother root. DBase is approximated by the distance between the parent root base and the point located on the mother root that is closest to the point starting a lateral root. Using RSML files, only RSA data associated with roots possessing a branching order lower or equal to 5 are used for the computation of RSA traits.
Growth rates are calculated as follows:
Firstorder root growth rate: for the first observation date, it is calculated as the ratio of the total firstorder root length to the root system age. For other observation dates (t), it is calculated as the difference between the total firstorder root length at time t and t1 divided by the difference between the root system age at time t and t1.
Lateral root growth rates: for the first observation date, it is calculated as the ratio of the lateral root length to the root system age. For other observation dates (t), it is calculated as the difference between the lateral root length at time t and t1 divided by the difference between the root system age at time t and t1.
Growth rate of the root system: for the first observation date, it is calculated as the ratio of the total root system length to the root system age. For other observation dates (t), it is calculated as the difference between the total root system length at time t and t1 divided by the difference between the root system age at time t and t1.
Returns a data frame summarizing all RSA traits calculated for each rac/RSML file located in inputrac
/inputrsml
.
The data frame contains the following columns: FileName (the name of the rac/RSML files), Time (the root system age expressed in the same unit (=unittime) as in the tps file(s) (for rac files) or in the same unit as rsml.date
(for RSML files)), TRL (the total root system length expressed in unitlength
), GRTR (the growth rate of the root system expressed in unitlength
/unittime), L1R (the firstorder root length expressed in unitlength
), GR1R (the firstorder root growth rate expressed in unitlength
/unittime), TN1R (the total number of firstorder roots), TNLR (the total number of lateral roots), TLRL (the total lateral root length expressed in unitlength
), NxLR (the total number of lateral roots with a branching order equal to x), LxLR (the total length of lateral roots with a branching order equal to x expressed in unitlength
), MLxLR (the mean length of lateral roots with a branching order equal to x expressed in unitlength
), GRxL (the growth rate of lateral roots with a branching order equal to x expressed in unitlength
/unittime), D2LR (the density of secondary roots on the firstorder root(s) expressed in root(s)/unitlength
).
Benjamin M. Delory, Caroline Baudson, Yves Brostaux, Guillaume Lobet, Loic Pages, Patrick du Jardin, Pierre Delaplace
Delory B.M., Baudson C., Brostaux Y., Lobet G., du Jardin P., Pages L., Delaplace P. (2016) archiDART: an R package for the automated computation of plant root architectural traits, Plant and Soil, DOI: 10.1007/s1110401526734.
Le Bot J., Serra V., Fabre J., Draye X., Adamowicz S., Pages L. (2010) DART: a software to analyse root system architecture and development from captured images, Plant and Soil, DOI: 10.1007/s1110400900052.
Lobet G., Pound M.P., Diener J., Pradal C., Draye X., Godin C., Javaux M., Leitner D., Meunier F., Nacry P., Pridmore T.P., Schnepf A. (2015) Root System Markup Language: Toward a Unified Root Architecture Description Language, Plant Physiology, DOI: 10.1104/pp.114.253625.
Pages L., Becel C., Boukcim H., Moreau D., Nguyen C., Voisin, AS. (2013) Calibration and evaluation of ArchiSimple, a simple model of root system architecture, Ecological Modelling, DOI: 10.1016/j.ecolmodel.2013.11.014.
1 2 3 4 5 6 7 8 9 10 11 12  ## Locate folder with DART and RSML files
path < system.file("extdata", package="archiDART")
## Compute RSA parameters for DART files only
res2a < architect(inputrac=path, inputtps=path, res=75, unitlength="cm")
## Compute RSA parameters for RSML files only
res2b < architect(inputrsml=path, unitlength="cm", rsml.connect=TRUE, rsml.date="age")
## Compute RSA parameters for DART and RSML files
res2c < architect(inputrac=path, inputtps=path, inputrsml=path, res=75, unitlength="cm",
rsml.connect=TRUE, rsml.date="age")

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