crispRdesignR: Guide Sequence Design for CRISPR/Cas9

Designs guide sequences for CRISPR/Cas9 genome editing and provides information on sequence features pertinent to guide efficiency. Sequence features include annotated off-target predictions in a user-selected genome and a predicted efficiency score based on the model described in Doench et al. (2016) <doi:10.1038/nbt.3437>. Users are able to import additional genomes and genome annotation files to use when searching and annotating off-target hits. All guide sequences and off-target data can be generated through the 'R' console with sgRNA_Design() or through 'crispRdesignR's' user interface with crispRdesignRUI(). CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the associated protein Cas9 refer to a technique used in genome editing.

Getting started

Package details

AuthorDylan Beeber [aut, cre], Frederic Chain [aut]
MaintainerDylan Beeber <dylan.beeber@gmail.com>
LicenseGPL-3
Version1.1.5
URL <https://github.com/dylanbeeber/crispRdesignR>
Package repositoryView on CRAN
Installation Install the latest version of this package by entering the following in R:
install.packages("crispRdesignR")

Try the crispRdesignR package in your browser

Any scripts or data that you put into this service are public.

crispRdesignR documentation built on July 1, 2020, 10:01 p.m.